Sprite Sheet Producer
A quick and easy way to create sprite sheets of uniform sized images. Simply add in the images you want on the sprite sheet (providing they are all of equal sizes) and Sprite Sheet Producer will quickly create a single graphic file for use elsewhere. Use PNG images with transparency and the final sprite sheet will maintain the transparency of each sprite.
You can even tweak the sprites position if you need to, specify an alternative width and height of the sprites, change the number of sprites in each row, and offset the graphic/image within each sprite.
Take a look through the features listing and the user guide to see if it meets your needs.